PerkinElmer Scintillation Cocktails & Consumables

PerkinElmer

Scintillation Cocktails & Consumables

For every liquid scintillation

counting application

Total solutions for liquid scintillation counting

applications — from PerkinElmer

Address any liquid scintillation counting need with PerkinElmer’s liquid

scintillation cocktails, tissue solubilizers and specialty chemicals. You can be

confident that our products meet the highest standards of performance, user

safety, environmental safety, and user convenience. Plus, our portfolio of

application notes can assist you with even the most complex liquid scintillation

counting applications you perform in your research.

Combine our scintillation cocktails with our trusted NEN

Radiochemicals, high

quality counting vials and market-leading radiometric detection instrumentation

for a complete single-source solution for your radiometric application needs.

Plus, no matter where your lab is, our global distribution network will ensure that

the products you order reach you reliably and on time. You just won’t find a better

source of products and services to support your research than PerkinElmer.

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Scintillation Cocktail Comparison Chart .............................................................................................4

Safer LSC Cocktails ............................................................................................................................6

Ultima Gold Family .................................................................................................................................6

OptiPhase HiSafe Famil

y ......................................................................................................................10

Opti-Fluor Family..................................................................................................................................11

Other Safer LSC Cocktails ....................................................................................................................12

Classical LSC Cocktails ....................................................................................................................13

Emulsifying Cocktails (Pico-Fluor Family, Filter-Count, Hionic Fluor) .......................................................13

Other Classical Cocktails (Aquasol-2,

Aquassure, Atomlight, Biofluor,

Econofluor-2, Insta-Fluor Plus, Insta-Gel Plus).......................................................................................15

Sample Oxidizer Cocktails (Carbo-Sorb E, Monophase S, Permafluor E

)...............................................17

Flow Detection Cocktails ..................................................................................................................18

Ultima-Flo Safer Flow Detection Cocktail Family....................................................................................18

Flo-Scint Classical Flow Detection Cocktail Famil

y ................................................................................20

Cocktails & Scintillators for Microplate Formats ............................................................................21

MicroScint LSC Cocktails......................................................................................................................21

Meltilex Solid Scintilla

tors .....................................................................................................................22

Tissue Solubilizers ............................................................................................................................23

Safer & Classical Tissue Solubilizers (SOLVABLE,

Soluene-350) ............................................................23

Specialty Chemicals .........................................................................................................................25

LIPIDEX ................................................................................................................................................25

Autoradiog

raphy Enhancers (EN

HANCE, ENLIGHTNING) .......................................................................25

Decontaminants & Cleaners (AbSolve, COUNT-OFF, Pico-Kleen N) ........................................................26

Liquid Scintillation Counting Vials ....................................................................................................27

Miniature Vials (Pico Prias, Pico ‘Hang-In’, Pico Glass,

Pony, Pony ‘Hang-In’, Polypropylene)..................28

Mid-Sized Vials (Hinge Cap, Midi, Maxi) ................................................................................................31

Standard Vials (Super & Low Diffusion Polyethylene, High Performance Glass, Econo Glass, Oximate)...32

QUANTULUS Vials (Teflon).....................................................................................................................35

Vials Selection Guide.........................................................................................................................36

Coc

ktail Selection Guides..................................................................................................................37

LSC Selection Guide by Filter Type........................................................................................................37

LSC Selection Guide b

y Sample Type....................................................................................................38

Flow Cocktails Selection Guide.........................................................................................................43

Sample Pr

eparation Guide ................................................................................................................44

Contents

Scintillation Cocktail Comparison

The table below provides information to help you choose the correct cocktail. This depends on the

nature of the sample to be counted as well as the sample preparation procedure and instrument used.

Only the right cocktail choice, paired with a correct sample preparation procedure and instrument,

will result in accurate and reproducible counting results.

Typical counting efficiencies determined on a PerkinElmer Tri-Carb 3100TR/LL (preset

H region, 0–18.6 keV).

Typical maximum sample volume (mL) per 10 mL cocktail at 20 ºC.

Ultima Gold with tissue solubilizers, preferably counted within 24 hours.

Ultima Gold DIPN ~150 56 52 49

Ultima Gold XR DIPN ~150 50 46 N.A.

Ultima Gold LLT DIPN ~140 52 46 N.A.

Ultima Gold MV DIPN ~110 57 55 N.A.

Ultima Gold AB DIPN ~140 52 46 N.A.

HiSafe 2 DIPN ~150 56 52 49

HiSafe 3 DIPN ~150 50 46 N.A.

Opti-Fluor Linear Alkylbenzyne ~150 44 40 N.A.

Emulsifier-Safe Linear Alkylbenzyne ~150 43 39 N.A.

Insta-Gel Plus Pseudocumene 48–50 56 48 N.A.

Pico-Fluor 15 Pseudocumene 48–50 57 53 N.A.

Pico-Fluor 40 Pseudocumene 48–50 51 45 N.A.

Filter-Count Pseudocumene 48–50 57 53 N.A.

Hionic-Fluor Pseudocumene 48–50 51 45 48

Ultima Gold F DIPN ~150 65 N.A. N.A.

BetaPlate Scint DIPN ~150 65 N.A. N.A.

Opti-Fluor O Linear Alkylbenzyne ~150 59 N.A. N.A.

Insta-Fluor Plus Pseudocumene 48–50 65 N.A. 57

COCKTAILS FOR AQUEOUS SAMPLES

ORGANIC SAMPLES

Counting Efficiency, %

Type of Flashpoint No With With

Cocktail Solvent °C/Tag CC Sample 10% Water 10% Solubilizer W

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PerkinElmer has a large collection of Application Notes that can help you with sample preparation.

Please visit our website (www.perkinelmer.com/cocktails) or contact Technical Support for assistance.

* = limited capacity - = no capacity

3.2 3.0–6.0 3.0–5.0 2.0–4.0 1.0–4.0 0–3.0

10.0 8.0–10.0 8.0–10.0 5.0–8.0 3.0–7.0 0–5.0

12.0 Optimized for all water types.

1.0 1.0–3.0 2.0–4.0 2.0–4.0 0–2.0 *

10.0 Optimized for 1–2 M mineral acids.

3.2 3.0–6.0 3.0–5.0 2.0–4.0 1.0–4.0 0–3.0

10.0 8.0–10.0 8.0–10.0 5.0–8.0 3.0–7.0 0–5.0

1.5 1.5–2.5 1.5–2.5 0.5–1.0 * -

1.5 1.5 1.5 1.0–1.5 0.5–1.0 -

1.8 and 3–10 1.8 and 3–10 1.8 and 3–10 0.5–1.0 and 3–10 0.5–1.5 0.1–1.5

1.5 1.5–2.0 1.5–2.5 0.5–1.0 - -

3.0 1.5–2.0 5.0–10.0 2.0–2.4 1.0–2.0 0.5–1.0

1.0 * * * * *

1.0 * * 1.0–1.5 1.5–2.5 1.5–4.0

For organic samples and dried filter membranes only.

For organic samples only.

Sample Load Capacity, mL

Water 0–0.05 M 0.05–0.2 M 0.2–0.5 M 0.5–1.0 M Over 1.0 M

Occupational safety in laboratories is of unques-

tioned importance. Traditional scintillation cocktail

formulations contain flammable, toxic solvents that

permeate through polyethylene and may represent

a hazard to laboratory workers, create disposal

problems that place strains on the environment,

and often add hidden lab costs. PerkinElmer has

addressed this problem by offering several lines

of safer LSC cocktails.

Ultima Gold Family

Beginning in the early 1980’s, user and environmental safety concerns led to the introduction of cocktails

based on high flash point solvents. Research conducted by Packard BioSciences Corp., later acquired by

PerkinElmer, led to the development of the Ultima Gold

™

family, high performance cocktails with the

following properties:

Safer LSC Cocktails

Typical sample load capacity of Ultima Gold with various sample types

Ultima Gold

Ultima Gold is a safer liquid scintillation cocktail

for a wide range of aqueous and non-aqueous

samples. This multipurpose LSC cocktail has a

high counting efficiency and provides superior

detection efficiency for samples that exhibit

severe quench in conventional cocktails.

• Extremely high counting efficiency:

up to 56% for

• Universal cocktail for aqueous and

nonaqueous samples

• High quench resistance

• No diffusion through polyethylene vials

• Biodegradable

• High flashpoint of approximately 150 °C

• Safely used outside a fume hood

Very high flash point for simple transportation

and no special stor

age requirements

Very low vapor pressure; nonvolatile

Low toxicity

Biodegradable

“Harmless chemicals” classification;

nonflammable

High counting efficiency

High quench resistance

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Typical

H counting efficiency of Ultima Gold

0.15 M NaCl added to 10 mL Cocktail 0 mL 1 mL 2 mL

Ultima Gold 56% 52% 49%

Alkylbenzene-based LSC cocktails 44% 40% 38%

Tri-Carb 2250TR/LL with 67% absolute

H efficiency (sealed argon purged standard).

Ultima Gold AB

Specifically designed for alpha/beta discrimination in liquid scintillation counting, Ultima Gold AB

provides the slow pulse decay characteristics necessary for effective alpha/beta discrimination. An

excellent sample holding capacity makes it ideal for a variety of aqueous and acidic sample types.

Typical sample uptake and misc

lassification range of Ultima Gold AB at 20 °C

Type of Sample Ultima Gold AB 85% (v/v) UG AB 75% (v/v) UG AB 50% (v/v) UG AB

+ 15% (v/v) UG F

+ 25% UG F + 50% UG F

Water

Sample uptake range 0.2–10.0 mL 0.2–10.0 mL 0.2–2.25 mL 0.2–0.5 mL

Misclassification range 0.74–1.87% 0.70–1.74% 0.58–0.68% 0.42–0.47%

1 M HCl

Sample uptake range 0.2–5.5 mL 0.2–2.0 mL 0.2–1.25 mL 0.2–0.4 mL

Misclassification range 0.87–1.73% 0.64–0.79% 0.64–0.75% 0.43–0.52%

2 M HCl

Sample uptake range 0.2–2.25 mL 0.2–1.25 mL 0.2–1.0 mL 0.2–0.3 mL

Misclassification range 0.61–1.07% 0.56–0.76% 0.49–0.60% 0.38–0.49%

1 M HNO

Sample uptake range 0.2–3.25 mL 0.2–1.75 mL 0.2–1.25 mL 0.2–0.4 mL

Misclassification range 0.75–3.85% 0.66–1.51% 0.55–0.98% 0.48–0.53%

2 M HNO

Sample uptake range 0.2–2.25 mL 0.2–1.5 mL 0.2–1.0 mL 0.2–0.3 mL

Misclassification range 0.77–4.21% 0.60–1.91% 0.70–1.17% 0.54–0.62%

Typical percentage misclassifications using a Tri-Carb 2550TR/AB and Time-Resolved Pulse Decay Analysis. Sample uptake per 10 mL cocktail

at 20 °C. For dilute samples or smaller sample volumes, dilute Ultima Gold AB with Ultima Gold F to increase energy resolution and counting

efficiency, and further reduce misclassifica

tion of alphas as beta and betas as alpha.

Ultima Gold F

Ultima Gold F is a high efficiency cocktail for counting dry filter supports, as well as non-aqueous

(organic) samples. For alpha/beta LSC counting, Ultima Gold F is an ideal diluent for Ultima Gold AB,

increasing energy pulse-shape resolution for small volume samples.

Typical

H quench resistance of Ultima Gold F

CCI

added to 10 mL Cocktail 0 µL 10 µL 20 µL 30 µL 40 µL 50 µL

Tritium Counting Efficiency 63% 51% 45% 39% 34% 31%

Tri-Carb 2250TR/LL with 67% absolute

H efficiency (sealed argon purged standard).

Ultima Gold LLT

Determine low levels of

H in a wide range of water samples

without requiring distillation. It accepts up to 54% tap water,

river water, rain water, and even sea water, with

H counting

efficiencies of approximately 30% and with very low background

levels. When used with PerkinElmer’s Tri-Carb

Liquid

Scintillation Analyzers or the QUANTULUS

Ultra Low Level

Liquid Scintillation Spectrometer, minimum detectable activities

are less than 1.1 Bq/L (500 minute count time).

Typical

H counting efficiency of dried filters

Filter Type Counting Efficiency

Glass Fiber GF/C 38% (

H-Thymidine)

Glass Fiber GF/B 36% (

H-Thymidine)

Tri-Carb 2250TR/LL with 67% absolute

H efficiency (sealed argon purged standard).

Performance of Ultima Gold LLT with various types of water

Safer LSC Cocktails

Ultima Gold MV

Ultima Gold MV is specifically formulated for the rapid uptake of aqueous and non-aqueous samples.

It is recommended for counting wet or damp glass fiber filters from cell harvesters. It is also ideal for

counting small volume samples in miniature vials and microtubes because of its reduced viscosity

compared to other high flash point cocktails.

Performance of Ultima Gold LLT for low level

Optimized Window (0.4 – 4.5 keV)

Water to Cocktail Ratio Cocktail %

H Bkg. Eff. E

/B E

/B MDA (Bq/L

-1

)

8 mL:12 mL Ultima Gold LLT 24.6% 1.15 CPM 526 33680 1.22

10 mL:10 mL Ultima Gold LLT 21.2% 1.11 CPM 405 40490 1.11

11 mL :9 mL Ultima Gold LLT 18.1% 0.95 CPM 345 41730 1.06

Count Conditions: Tri-Carb 2770TR/SL operated at 15 °C, in Low Level Count Mode, all samples in duplicate, 500 minute count time.

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Typical sample load capacity of Ultima Gold MV with various sample types

Typical microvolume

H counting efficiency of Ultima Gold MV

0.15 M NaCl added to 1,000 µL Cocktail 0 µL 50 µL 100 µL 150 µL 200 µL

Tritium counting efficiency 57% 55% 54% 53% 52%

Tri-Carb 2250TR/LL with 67% absolute

H efficiency (sealed argon purged standard).

Ultima Gold XR

Ultima Gold XR is a safer liquid scintillation cocktail with a very high sample load capacity. Choose

it to count large sample volumes, or when using miniature vials to increase throughput, reduce cost

per sample or minimize radioactive waste. Ultima Gold XR is compatible with alkaline samples.

Typical sample load capacity of Ultima Gold XR

Safer LSC Cocktails

Product Product No. Size

Ultima Gold 6013326 1 x 5 L

6013327 4 x 2.5 L

6013329 2 x 5 L

6013322 1 x 25 L

Ultima Gold AB 6013301 1 x 1 L

6013309 2 x 5 L

Ultima Gold F 6013179 2 x 5 L

Ultima Gold LLT 6013371 1 x 1 L

6013377 4 x 2.5 L

Ultima Gold MV 6013151 1 x 1 L

6013159 2 x 5 L

Ultima Gold XR 6013117 4 x 2.5 L

6013119 2 x 5 L

6013112 1 x 25 L

Sample sizes of all products in table above are available. To order a sample, please contact your PerkinElmer Sales Representative.

OptiPhase HiSafe 2

OptiPhase HiSafe 2 is a general-purpose liquid

scintillation cocktail. It combines very high

counting efficiency with moderate to high sample

holding capacity for a wide range of aqueous and

non-aqueous solutes.

OptiPhase HiSafe 3

OptiPhase HiSafe 3 is a liquid scintillator that

handles a broad range of solutes. Used for a

variety of scintillation applications, it combines

good counting efficiency with a very high level

of sample acceptance, particularly for high ionic

strength solutes.

Typical

H quench resistance of Ultima Gold XR

Water added to 10 mL Cocktail 0 mL 1 mL 2 mL 5 mL 10 mL

Tritium counting efficiency 50% 46% 43% 37% 29%

Tri-Carb 2250TR/LL with 67% absolute

H efficiency (sealed argon-purged standard).

OptiPhase HiSafe Family

A major technological advance in safer LSC cocktails, our OptiPhase HiSafe family of safer LSC cocktails uses

the solvent di-isopropylnaphthalene (DIN) to achieve improved safety without decreasing performance.

High flashpoint (148 °C)

Low vapor pressure (1 mm Hg at 25 °C)

Virtually odorless and colorless

Low toxicity and irritancy (LD

5,600 mg/kg)

No permeation through plastic vials

Low photo- and chemiluminescence

High

H counting efficiency

Good color and quench resistance

No adverse toxicological effects

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Product Product No. Size

OptiPhase HiSafe 2 1200-436 1 x 5 L

OptiPhase HiSafe 3 1200-437 1 x 5 L

OptiPhase SuperMix 1200-439 1 x 5 L

Betaplate Scint 1205-440 1 x 5 L

OptiScint HiSafe 1200-434 1 x 5 L

OptiPhase SuperMix

OptiPhase SuperMix has been specially formulated

for use with microplates. It mixes easily with a

wide variety of aqueous solutes and has a very

high uptake capacity, minimizing cocktail use

and reducing disposal problems.

OptiScint HiSafe

OptiScint HiSafe is an environmentally safe DIN-

based scintillation cocktail with high counting

efficiency, suitable for all non-aqueous samples.

Betaplate Scint

Betaplate Scint is a HiSafe cocktail for samples

harvested or spotted onto dry filter membranes.

High counting efficiency may eliminate sample

pre-treatment often necessary with conventional

cocktails. Ideal for use with samples in organic

solutions.

Typical sample load capacity of Opti-Fluor with various sample types

Opti-Fluor Family

Our Opti-Fluor

cocktails are universal, safer liquid scintillation

cocktails designed for use with polyethylene vials.

Multipurpose liquid scintillation cocktail

Low photo- and chemiluminescence

No diffusion through polyethylene vials

Opti-Fluor

Based on the high flash point solvent LAB (linear alkyl benzene), Opti-Fluor does not show any

diffusion through the walls of polyethylene vials (observed with many LSC cocktails containing

toluene, xylene or pseudocumene).

Biodegradable

w toxicity, increasing occupational safety

High flashpoint of approximately 150 °C

Safer LSC Cocktails

Product Product No. Size

Opti-Fluor 6013199 2 x 5 L

Opti-Fluor O 6013339 2 x 5 L

Sample sizes of all products in table above are available. To order a sample, please contact your PerkinElmer Sales Representative.

Other Safer LSC Cocktails

Emulsifier-Safe

Emulsifier-Safe™ is a LAB-based cocktail that is

economically priced for aqueous and organic

samples. Aqueous samples and many buffer

solutions are accepted in a single liquid phase

up to a 10 to 15% sample load.

Formula-989

is a high flash point, nonflammable

LAB-based cocktail that is designed for benchtop

use. Formula-989 can accept a variety of aqueous

samples up to 20 to 30%, by volume, as stable

homogeneous mixtures.

High Efficiency Mineral Oil Scintillator

High Efficiency Mineral Oil Scintillator is the

cocktail of choice for the detection of radon in

water and soil samples. It yields a high counting

efficiency and provides the same accuracy as

solid detectors for radon. It has a low volatility

and a high flash point (79 °C/175 °F).

Product Product No. Size

Emulsifier-Safe 6013389 2 x 5 L

Formula-989 6NE9899 2 x 5 L

High Efficiency Mineral Oil Scintillator 6NE9571 1 x 1 L

6NE9579 4 x 2.5 L

Opti-Fluor O

Opti-Fluor O is used for counting organic (non-aqueous) samples. It will accommodate many organic

solvents, forming clear liquid solutions yielding good counting efficiencies.

Opti-Fluor O can replace classical toluene-, xylene- or pseudocumene-based LSC cocktails for organic

samples. Opti-Fluor O is ideally suited for counting radon in water when a safer cocktail is preferred.

Compatibility of Opti-Fluor O with different solvents

Sample type Compatibility

Acetic Acid Yes*

Acetone Yes

Acetonitrile No

Chloroform Yes

Dichloromethane Yes

Dimethyl Sulfoxide No

Sample type Compatibility

Ethanol–96% No

Ethanol–100% Yes

Ethyl Acetate Yes

Ethylene Glycol No

Heptane Yes

Methanol No

*Maximum capacity approximately 4% sample load; for all other sample types up to 50% capacity is recommended.

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Classical LSC Cocktails

Pico-Fluor 15

Pico-Fluor 15 is specifically formulated to provide accurate and reproducible high efficiency counting

of aqueous samples. It will accept up to 2 mL of a wide variety of aqueous samples in 10 mL of cocktail.

The continuous single liquid phase, formed from zero to the maximum acceptable sample load, avoids

unexpected two-phase separation.

PerkinElmer offers classical liquid scintillation

cocktail formulations that are optimized for

the highest counting efficiency and maximum

sample holding capacity. Our prepared cock-

tails are easy-to-use, save preparation time

and minimize laboratory errors. Our carefully

controlled blending and quality assurance

procedures provide high performance, batch

homogeneity and lot-to-lot uniformity.

Emulsifying Cocktails

Pico-Fluor Family

The Pico-Fluor

™

family is a range of pseudocumene-based liquid scintillation counting cocktails.

Pico-Fluor 30

Pico-Fluor 30 is a complete, ready-to-use cocktail specially formulated for counting high sample loads

of aqueous solutions. It excels in the incorporation of samples such as phosphate-buffered saline with

sample loads often exceeding 30%.

Typical sample load capacity of Pico-Fluor 15 with various sample types

Classical LSC Cocktails

Pico-Fluor 40

Pico-Fluor 40 is a universal cocktail for use with both conventional 20 mL size vials and miniature

vials. It has a large sample holding capacity and high quench resistance and is compatible with

tissue solubilizers.

Filter-Count

Filter-Count™ is specifically formulated to dissolve cellulose nitrate membrane filters. It can also dis-

solve mixed cellulose esters and polyvinyl chloride (PVC) filters, although these sample types may

require additional time. Filter-Count can be used for wet or dry filter counting, reducing sample prepa-

ration procedures and improving counting results by enabling the use of external standard quench

monitoring.

Hionic-Fluor

Hionic-Fluor™ is a cocktail for samples with high ionic

strength and solubilized samples in strong alkaline media.

ionic-Fluor exhibits extremely fast chemiluminescence

decay with alkaline solutions or tissue solubilizers such

as Soluene

-350 and SOLVABLE™.

Typical sample load capacity of Pico-Fluor 40 with various sample types

Decay of Chemiluminescence in Hionic-Fluor

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Other Classical Cocktails

Aquasol-2

Aquasol™-2 is a second generation, universal

LSC cocktail that improves and extends the

features of its predecessor cocktail, Aquasol.

It is xylene based and is suitable for difficult-to-

count samples. Aquasol-2 is a gelling cocktail

and yields high counting efficiencies while still

accepting water samples up to 50% loading.

Aquassure

is a gelling pseudocumene-based

LSC cocktail and is suitable for counting a wide

range of samples with great flexibility in sample

volume. Aquassure has the benefits of reduced

permeation through polyethylene, higher tritium

counting efficiency and lower vapor pressure.

Atomlight

is a pseudocumene-based LSC cock-

tail that is ideal for counting high salt concentra-

tion aqueous samples. It holds the maximum

amount of sample in the minimum amount of

cocktail and is ideal for use with miniature vials.

Biofluor

is a pseudocumene-based LSC cocktail

ideal for counting low to intermediate volumes

of aqueous samples. It is a high efficiency

monophasic cocktail that will accommodate up

to 2 mL of aqueous sample in 15 mL cocktail.

Typical sample load capacity of Hionic-Fluor with various sample types

Product Product No. Size

Filter-Count 6013141 1 x 1 L

6013149 2 x 5 L

Hionic-Fluor 6013311 1 x 1 L

6013319 2 x 5 L

6013312 1 x 20 L

Pico-Fluor 15 6013059 2 x 5 L

Pico-Fluor 30 6013049 2 x 5 L

Pico-Fluor 40 6013349 2 x 5 L

A sample size of Hionic-Fluor is available. To order a sample, please contact your PerkinElmer Sales Representative.

Typical phase diagram of Insta-Gel Plus for water

Note: For low level counting of distilled water samples, Ultima Gold LLT is recommended.

Classical LSC Cocktails

Product Product No. Size

Aquasol-2 6NE9529 2 x 5 L

Aquassure 6NE9659 2 x 5 L

Atomlight 6NE9689 2 x 5 L

Biofluor 6NE9619 2 x 5 L

Econofluor-2 6NE9699 4 x 2.5 L

Insta-Fluor Plus 6013121 1 x 1 L

6013127 4 x 2.5 L

Insta-Gel Plus 6013391 1 x 1 L

6013399 2 x 5 L

Econofluor-2

Econofluor

-2 is a pseudocumene based LSC

cocktail blended for optimal counting of organic

samples and non-aqueous solutions. Econofluor-2

is compatible with most organics, including

acetic and fatty acids, lipids, steroids, fats,

terpenes and prostaglandins. Econofluor-2 is also

ideally suited for use in two-phase extraction

assays (e.g., CAT assays).

Insta-Fluor Plus

Insta-Fluor™ Plus is a pseudocumene-based

cocktail blended for optimal counting of organic

samples and non-aqueous solutions. Simply

combine the organic sample with Insta-Fluor

Plus, shake to ensure homogeneity and count.

Insta-Fluor Plus is ideally suited for use in two-

phase extraction assays (e.g., CAT assays).

Insta-Gel Plus

Insta-Gel Plus is the

pseudocumene-based

cocktail of choice for

a large variety of

applications. It excels

in the incorporation

of water and aqueous-

soluble samples and

is equally useful for

organic-soluble sam-

ples. Due to the very

high sample holding

capacity and its typical gel formation, Insta-Gel

Plus is ideal for counting large volumes of water,

TLC scrapings and suspended solids.

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Sample Oxidizer Cocktails

These pseudocumene-based oxidizer cocktails are designed

for use with PerkinElmer’s Sample Oxidizers to ensure superior

performance and consistently reliable results.

Carbo-Sorb E

Carbo-Sorb

E is a high capacity radioactive carbon

dioxide absorber compatible with the counting cocktail

Permafluor

Monophase S

Monophase

S is specifically formulated for counting

pure water samples. It will accept up to 23% water,

forming a clear fluid that yields outstanding counting

efficiencies. It does not foam and does not form a gel,

even at extreme mixing ratios (washing cycle). It is the

cocktail of choice for obtaining the highest

H counting

performance from Sample Oxidizers.

Permafluor E

is uniquely designed for counting

samples that are trapped in Carbo-Sorb E.

Product Product No. Size

Carbo-Sorb E 6013721 1 x 1 L

6013729* 4 x 2.5 L

Monophase S 6003043 1 x 1 L

6013107 4 x 2.5 L

6013109 2 x 5 L

Permafluor E

6013181 1 x 1 L

6013187 4 x 2.5 L

* Product No. 6013729 is only available for shipment within Europe.

PerkinElmer Sample Oxidizer

Flow Detection Cocktails

Ultima-Flo AF

Ultima-Flo AF is formulated to accept gradients

up to 2.0 M ammonium formate at a 1:1 ratio

with fast and easy mixing. It is the cocktail of

choice when using ammonium formate buffers

to elute radiolabeled inositol phosphates from

HPLC columns.

Our flow detection cocktails are specially designed for use with PerkinElmer’s Flow Scintillation Analyzer.

Ultima-Flo Safer Flow Detection Cocktail Family

The Ultima-Flo

™

family includes three novel, biodegradable cocktails for use in flow scintillation analyzers.

The high loading capacity of the Ultima-Flo cocktails means less cocktail required, longer residence times

and higher sensitivity.

The Ultima-Flo series can replace the complete family of classical flow cocktails. Ultima-Flo cocktails outper-

form the classical cocktails on mixing ratio (up to 1:1). They also provide the user with the safety features

related to the high flash point solvent system.

Typical sample load capacities of Ultima-Flo AF for 0–2.0 M ammonium formate gradient (pH 3.8)

Ultima-Flo AF performance with 0–2.0 M ammonium

formate gradient (pH 3.8)

Ultima-Flo M

Ultima-Flo M is formulated for multipurpose flow counting applications. This safer cocktail has a high

sample acceptance for a wide range of dilute HPLC eluents, and methanol and acetonitrile gradients.

Ultima-Flo M has low viscosity, unique rapid mixing properties and is resistant to chemiluminescence.

Ultima-Flo M performance with various samples at different ratios of cocktail to sample

Typical sample load capacities of Ultima-Flo AP for 0–2.0 M ammonium phosphate gradient (pH 3.8)

Ultima-Flo AP performance with 0–2.0 M ammonium

phosphate gradient (pH 3.8)

Performance for Ultima-Flo AP for other sample types

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Ultima-Flo AP

Ultima-Flo AP accepts gradients up to 2.0 M ammonium phosphate with fast and easy mixing. It

demonstrates high counting efficiency and quench resistance for a wide variety of sample types.

Product Product No. Size

Ultima-Flo AF 6013589 2 x 5 L

Ultima-Flo AP 6013599 2 x 5 L

6013592 1 x 20 L

Ultima-Flo M 6013579 2 x 5 L

6013572 1 x 20 L

Sample sizes of Ultima-Flo AP and Ultima-Flo M are available. To order a sample, please contact your PerkinElmer Sales Representative.

Flow Detection Cocktails

Deionized water 50.0% 1:1

Methanol/Water (50/50) 31.0% 3:1

Methanol 50.0% 1:1

Acetonitrile/Water (50/50) 41.2% 2:1

Acetonitrile 50.0% 1:1

0.2 M NaCl 41.2% 2:1

0.05 M NaCl 50.0% 1:1

0.1 M PBS 33.3% 2:1

0.01 M PBS 41.2% 2:1

0.01 M PBS/plasma (10%) 45.9% 2:1

1.0 M NaOH 21.6% 4:1

0.5 M NaOH 35.5% 2:1

0.1 M NaOH 50.0% 1:1

0.2 M HEPES 50.0% 1:1

0.1 M HEPES 50.0% 1:1

50 mM Tris-HCl 50.0% 1:1

0.05 M Na

HPO

50.0% 1:1

0.02 M Ammonium formate 50.0% 1:1

Typical sample load capacities for Ultima-Flo M (at 20 °C)

Sample Type Maximum Optimal

Sample Mixing Ratio

Uptake Cocktail:Sample

Sample Type Maximum Optimal

Sample Mixing Ratio

Uptake Cocktail:Sample

Flo-Scint Classical Flow Detection Cocktail Family

The classical Flo-Scint

™

cocktails are pseudocumene-based flow cocktails that have gained an excellent

reputation when gradients, especially those with methanol and acetonitrile, need to be counted. They are

resistant to chemiluminescence and are non-gelling.

Flo-Scint II

Flo-Scint II can be used with polar solvents and

dilute buffers affording good sample loading

capacity and low viscosity.

Flo-Scint III

Flo-Scint III is designed to work with methanol

and acetonitrile gradients as well as moderately

buffered solutions, including phosphates. It has

good sample loading capacity, low viscosity and

high counting efficiency.

Flo-Scint A

Flo-Scint A is suitable for acetonitrile/water

gradients, for counting polar organic solvents

and dilute salt solutions. It has excellent sample

loading capacities at up to 50% in aqueous

solutions. Flo-Scint A is also resistant to chemilu-

minescence and has a high counting efficiency.

Product Product No. Size

Flo-Scint II 6013529 2 x 5 L

Flo-Scint III 6013539 2 x 5 L

Flo-Scint A 6013569 2 x 5 L

www.perkinelmer.com

Cocktails & Scintillators for Microplate Formats

MicroScint LSC Cocktails

Specifically formulated for use with PerkinElmer’s TopCount Microplate Scintillation and

Luminescence Counter, MicroScint

™

cocktails mix easily with samples for rapid uptake of

aqueous and non-aqueous samples in microplates. These safer cocktails provide you

with a choice of optimal characteristics, including excellent counting efficiency, high

sample capacity, quench resistance and polystyrene compatibility.

MicroScint-20

MicroScint-20 cocktail accepts dilute aqueous

samples at up to 20% loading (up to 25 µL in

100 µL MicroScint-20). With these sample types,

MicroScint-20 cocktail mixes easily and com-

pletely upon agitation with an orbital shaker.

It is the cocktail of choice for counting filters

that have not been completely dried.

MicroScint-20 has an absolute, unquenched

tritium efficiency of approximately 52% when

measured in a 24-well white polystyrene

OptiPlate™ microplate.

MicroScint-40

MicroScint-40 cocktail accepts dilute aqueous

samples at up to at least 40% loading (up to

70 µL in 100 µL MicroScint-40). It also mixes

easily with most sample types but slightly longer

agitation may be necessary when handling large

sample volumes or more concentrated samples.

MicroScint-40 has an absolute, unquenched

tritium efficiency of approximately 40% when

measured in a 24-well white polystyrene

OptiPlate microplate.

MicroScint-E

MicroScint-E cocktail is used for assays that

require in situ partitioning of the radionuclide-

containing lipid phase from the aqueous phase

in microplates. This cocktail extracts the lipids

or other non-polar compounds from the aqueous

phase in such assays, enabling direct counting

of the samples after cocktail addition, since

the label is preferentially taken up into the

lipophilic cocktail.

MicroScint-E cocktail has an

absolute, un-quenched tri-

tium efficiency of approxi

mately 50% when measured

in a 24-well white poly-

styrene OptiPlate microplate.

MicroScint-O

MicroScint-O cocktail is used for counting non-

polar organic samples such as hexane, heptane,

ethyl acetate, etc., and for dried filters. It is

particularly useful for organic samples produced

from enzyme inhibition assays. MicroScint-O

cocktail does not contain surfactants and is

not miscible with water, so it is unsuitable

for counting aqueous samples.

MicroScint-O cocktail has an absolute, un-

quenched tritium efficiency of approximately

58% when measured in a 24-well white poly-

styrene OptiPlate microplate.

MicroScint-PS

MicroScint-PS cocktail, specifically formulated

for polystyrene compatibility, is suitable for

use with virtually any type of microplate and

provides high counting stability with minimal

change in count rate within a 24 hour period. It

has almost identical sample handling capabilities

to MicroScint-20 with respect to sample concen-

tration and loading, but has a lower viscosity

and is therefore easier and quicker to dispense.

MicroScint-PS cocktail has an absolute, un-

quenched tritium efficiency of approximately

48% when measured in a 24-well white poly-

styrene OptiPlate microplate.

Product Product No. Size

MicroScint-20 6013621 1 x 1 L

MicroScint-40 6013641 1 x 1 L

6013647 4 x 2.5 L

MicroScint-E 6013661 1 x 1 L

MicroScint-O 6013611 1 x 1 L

MicroScint-PS 6013631 1 x 1 L

Meltilex Solid Scintillators

Meltilex

melt-on solid scintillator, for use with filtermat-harvested or dot-blotted samples, is an

attractive alternative to traditional scintillation cocktails. Meltilex is especially well suited for use

with plate counters. Simply place a sheet of Meltilex and a filtermat containing 96 samples together.

Run these through a heat sealer or heat on a hot plate to melt. Sample preparation takes only one or

two minutes per filtermat.

Easy to use: simply melt onto a filtermat, completely encapsulating the samples.

Fixes samples in position:

eliminates the risk of sample migration or partial elution into cocktail.

Ensures stable counting conditions and long-term storage.

Reduces waste disposal costs: produces no liquid waste.

Reduces consumables costs: carrier vials can be reused.

Versatile: use with glass fiber nitro-cellulose or nylon filtermats with no increase in non-specific binding.

Available in a variety of sizes and thicknesses.

Typical Relative Counting Efficiency using Meltilex

Compared to Scintillation Cocktail

Isotope Efficiency (%)

Cr 61

H75

125

I78

C 100

Ca 97

P 100

Cocktails & Scintillators for Microplate Formats

Product Product No. Size

Meltilex A melt-on scintillator for Betaplate 1205 1205-441 100/pack

Meltilex B melt-on scintillator for Betaplate 1205 1205-442 75/pack

Meltilex B melt-on scintillator for Betaplate 1204 1204-442 75/pack

Meltilex A melt-on scintillator for MicroBeta 1450 1450-441 100/pack

Meltilex B melt-on scintillator for MicroBeta 1450 1450-442 75/pack

www.perkinelmer.com

Tissue Solubilizers

OptiSolv

OptiSolv is a strong organic base, formulated with toluene, which has an excellent capability for the

solubilization of biologicals and plants into a solution compatible with liquid scintillation cocktails.

Ideal for tissue homogenates, whole blood and high water content samples such as plasma,

brain and polyacrylamide gels.

Solubilization rate can be increased by warming up to 50–60 °C.

Minimum chemiluminescence produced.

Ideal for counting with Hionic-Fluor and Ultima Gold.

Product Product No. Size

SOLVABLE 6NE9100 500 mL

Safer Tissue Solubilizers

SOLVABLE

™

is an aqueous-based solubilizer that has an excellent capacity for the solubilization of wet

tissue, aqueous tissue homogenates, proteins, nucleotides and other substances into a solution. With the

exception of plant material, SOLVABLE can replace the classical solubilizer Soluene-350 for many

applications, increasing safety in the laboratory due to its aqueous nature. SOLVABLE is compatible with

Ultima Gold and has a superior mixing ratio with safer cocktails (2.5 mL sample in 10 mL cocktail).

Elutes samples from both agarose and polyacrylamide gels.

Fast digestion of whole tissue homogenates and whole blood.

Effectively captur

in enzymatic assays.

Low chemiluminescence produced.

Compatible with Ultima Gold and Hionic-Fluor.

Classical Tissue Solubilizers

Soluene-350

Soluene-350 is a strong organic base formulated with toluene. It has an excellent

capacity for the solubilization of wet tissue, aqueous tissue homogenates, proteins,

nucleotides, plant material and other substances into a solution compatible with liquid

scintillation cocktails. Compatible with Hionic-Fluor (4 mL sample in 10 mL cocktail)

and Ultima Gold (1 mL sample in 10 mL cocktail).

Product Product No. Size

Hyamine Hydroxide

10-X 6003005 500 mL

OptiSolv 1200-435 500 mL

Soluene-350 6003038 500 mL

6003037 2 x 2.5 L

Sample Preparation Techniques

Treatment Description Examples

1. Dissolving Dissolve sample directly into the Organic samples, e.g., lipids, steroids,

cocktail or use a co-solvent. etc. dissolved directly in LSC cocktails.

2. Emulsifying Aqueous samples counted in “surfactant- Various salt/buffer solutions, acids

containing” LSC cocktails (colloidal-solutions). and alkaline samples in ready-for-use

LSC cocktails.

3. Suspending Suspend insoluble particles in a stable gel phase. Soil samples in Insta-Gel Plus.

4. Extracting or Eluting Dissolving labeled compounds from TLC-scrapings, paper-chromatograms,

solid phase into solution. polyacrylamide gel slices, cellulose

nitrate filters.

5. Solubilizing Digesting tissue material (biological) using Cell material, feces, tissue samples

alkaline hydrolysis (tissue solubilizer). digested in Soluene-350 or SOLVABLE.

6. Wet Oxidation Digesting sample material with Digestion of plant material in nitric acid

strong acids and peroxides. or perchloric acid with hydrogen peroxide.

7. Combustion Combusting of dry and wet samples Absorption of

in Carbo-Sorb E,

with trapping of

and

O in Soluene-350 or Hyamine Hydroxide

;

suitable trapping agents.

O in Monophase S.

Tissue Solubilizers

Hyamine Hydroxide 10-X

Hyamine Hydroxide

10-X is a quaternary ammonium hydroxide solution. It can be used to

solubilize many biological tissues and as a

trapping agent.

Compatible with Insta-Fluor Plus (7.5 mL of Hyamine Hydroxide

saturated with carbon dioxide in

10 mL cocktail) and Emulsifier Safe (3 mL of Hyamine Hydroxide

saturated with carbon dioxide in

10 mL cocktail. The latter provides a safer system due to the high flash-point of this cocktail.

Solubilizes many biological tissues.

Resistant to c

hemiluminescence.

Recommended

trapping agent for helicobacter pylori and urea “breath” test studies.

Compatible with Emulsifier-Safe and Insta-Gel Plus.

www.perkinelmer.com

Specialty Chemicals

LIPIDEX

LIPIDEX products are lipophilic, hydrophobic column packing materials for liquid chromatography

used to separate a variety of steroids, prostaglandins, lipids and other natural products. LIPIDEX-1000

and LIPIDEX-5000 are alkoxylated derivatives of Sephadex

LH-20. Approximately 10% of the hydroxy

groups of LIPIDEX-1000 are substituted with long alkyl chains and in LIPIDEX-5000 the substitution is

approximately 50%. The polarity of LIPIDEX is a function of this substitution; thus LIPIDEX-1000 is

more polar than LIPIDEX-5000.

Product Product No. Size

LIPIDEX-1000 6008301 25 g

LIPIDEX-5000 6008303 25 g

HANCE Liquid Autoradiography

Enhancer

• Greatly simplifies gel processing as compared

to Bonner and Lasky’s method: requires no

dehydration, a short preparation time and

overall processing time is reduced from 5

hours to 90 minutes.

• Can be used for both polyacrylamide and

agarose gels: method of choice for agarose gels.

• Performs equivalent to PPO/DMSO for poly-

acrylamide gels, but eliminates gel distortion:

unlike PPO/DMSO, does not shrink gel.

• Contains no DMSO, odorless.

HANCE Spray Surface

Autoradiography Enhancer

• Designed for use with

H-,

C- and

S-labeled

compounds isolated on hybridization mem-

branes, TLC plates and similar solid supports.

• Significantly reduces exposure times and

permits the detection of extremely low

isotope levels.

• Non-reactive with x-ray film and most

samples and supports.

• Eliminates mixing of liquids and spillage.

• Not intended for use with tissue or gel

fluorography.

ENLIGHTNING Rapid

Autoradiography Enhancer

• Safer alternative for gels.

• Involves a single-step process: requires no

more than one-half hour to perform.

• Produces high-quality fluorograms: effective

with polyacrylamide and mixed gels.

• Store at room temperature: long shelf life.

• Contains no DMSO, odorless.

• All products are shipped and stored

at ambient temperature.

Autoradiography Enhancers

Product Product No. Size

HANCE

Autoradiography Enhancer 6NE9701 1 L

HANCE Spray Surface Autoradiography Enhancer 6NE970C 2 oz. (59 mL)

spray can

ENLIGHTNING

Rapid Autoradiography Enhancer 6NE9741 1 L

Specialty Chemicals

AbSolve Glassware Cleaner

• Easily and inexpensively removes serious

RNase or DNA contamination (autoclaving

does not destroy DNA). Simply soak plastic

and glassware in a 2% solution for 30 minutes,

rinse and use.

• Ideal for prewashing plastic tubes for PCR.

AbSolve™ prevents DNA contamination of

storage and reaction vessels from exogeneous

sources.

•

Use to clean glassware for electrophoresis.

Glass plates rinse easily and AbSolve leaves

no residue to interfere with gel polymerization

or silver staining.

• Safe, non-abrasive AbSolve does not etch glass,

contain strong acids, or emit toxic fumes.

COUNT-OFF Liquid Concentrate

• Ideal, all-purpose decontaminant: safe,

efficient and economical.

• Quickly and effectively cleans up even the

most persistent leftovers: proven most effective

cleanser for removing radioactive residues (

P and

125

I), stopcock and vacuum greases,

lanolin and petroleum jelly, dried blood

and serum, fatty and amino acids, protein

complexes, and polymer films and other

stubborn residues.

• Stable under extreme temperature fluctuations

(-50 °C to 150 °C).

• Safer than strongly acidic cleaners, such as

chromic acid: solutions will not produce toxic

gases from substrates containing

131

Cl.

• Easy to use: adjust concentration, soaking

time and temperature as needed to speed up

decontamination or for particularly stubborn

substances such as dried blood.

COUNT-OFF Surface Cleaner

• Quickly and safely decontaminates small

radioactive spills from benches, shields, and

appliances: also efficiently cleans instrument

housings, hood corners, centrifuge cups and

heads, and LSC counter mechanisms.

• Traps and suspends radioactive particles in

foam: makes clean-up easy and reduces the

likelihood of spreading contamination to

hands and clothing.

• Removes both ionic and non-polar radioactivity.

• Cleans-up stubborn grease, resins, blood and

wax, even from rough surfaces.

Pico-Kleen N

Pico-Kleen N is a concentrated liquid detergent

developed as an all-purpose cleaning agent

and radioactive decontaminant. Pico-Kleen N

is essentially neutral, in which mildness is

combined with an outstanding surfactant

formulation resulting in an effective cleaning

action. Depending on the type of radioactive

contaminated surface and isotopes, Pico-Kleen N

will effectively remove the contamination when

properly applied.

Product Product No. Size

AbSolve Glassware Cleaner 6NE9711 1 x 1 L

COUNT-OFF

™

Liquid Concentrate 6NE9422 1 x 2.5 L

6NE9427 4 x 2.5 L

COUNT-OFF Surface Cleaner 6NE942T 6 x 22 oz. (650 mL)

pump bottles

Pico-Kleen N 6013819 2 x 5 L

Decontaminants & Cleaners

Glass Vials

Glass vials are manufactured from low potassium

glass tubing. The tube diameter and the wall

thickness are very closely controlled. The

uniform wall thickness contributes to excellent

counting reproducibility.

Chemically inert: suitable for use with aggressive

eagents and tissue solubilizers.

Good visibility: to check sample/cocktail appearance.

No solvent permeation: when classical LSC

cocktails are used.

Choice of Caps

Foil-lined urea: best for airtight seal

Poly-cone line: best for aggressive reagents

such as alkalis and oxidizer reagents

Poly screw: basic plastic cap, no insert

Plastic Vials

Plastic vials are injection (blow) molded to

exacting specifications from virgin high-density

(linear) polyethylene (HDPE). Caps are recessed

to assure reliable loading and transferring in

automatic sample changers without skipping or

jamming. Since polyethylene vials are produced

from petrochemicals, they contain no measurable

background and are preferred for low activity

counting applications.

Lower background level than glass vials.

Higher counting efficienc

y than glass vials.

Combustible: easier waste disposal.

No solvent permeation with safer, high-flash point

cocktails such as the Ultima Gold and OptiPhase

HiSafe families.

PerkinElmer offers high quality glass and plastic vials. The best vial to choose is dependent on the type

and volume of sample to be counted and the cocktail that will be used.

www.perkinelmer.com

Liquid Scintillation Counting Vials

Pico Glass Vials

Pico Prias Vials

Miniature Vials

Pico Pro Vial - 4 mL

Our Pico Pro Vial™ is a 4 mL plastic scintillation

vial uniquely suited for use in cell harvesting

systems and general purpose LSC counting. A

push-on/stay-on cap provides fast closure of vials.

For use in the trays of cell harvesting systems,

the caps are connected in strings of six, with

spacing corresponding to the 6 x 16 formats of

the trays. After the glass fiber filters are com-

pletely dried, simply punch into Pico Pro V

ials

and add up to 4 mL scintillation cocktail, such

as Ultima Gold F. Lay a string of caps over a row

of six vials, and press the caps onto the vials

until a “click” is heard. The connections between

the caps are automatically broken, and the

remaining strings are folded upwards.

Specifications

Height with cap: 60.8 mm.

Diameter:

14.2 mm.

Diameter of opening: 11.2 mm.

Diameter of cap: 14.0 mm.

Wall thickness: 1.1 mm.

Nominal volume: 4.0 mL.

Maximum volume: 4.5 mL.

Temperature resistance: up to 80 °C.

Pico Prias Vial - 6 mL

Our polyethylene Pico Prias Vial™ yields

high counting efficiencies with 3– 6 mL of

LSC cocktail.

Specifications

Height with cap: 57.5 mm.

Diameter:

15.0 mm.

Diameter opening: 12.3 mm.

Diameter of cap: 16.2 mm.

Wall thickness: 1.3 mm.

Nominal volume: 6.0 mL.

Maximum volume: 6.5 mL.

Temperature resistance:

up to 80 °C.

Pico ‘Hang-In’ Vial - 6 mL

Our Pico ‘Hang-In’ Vial™ is a miniature polyeth-

ylene scintillation vial for use in standard 20 mL

liquid scintillation analyzers. The unique self-

centering design allows a 20 mL L

SC vial to be

used as a carrier. This system makes it possible to

use large and small vials (small vial contained in

a large vial) intermixed in one rack.

Specifications

Height with cap:

57.5 mm.

Diameter: 15.0 mm.

Diameter of opening: 12.3 mm.

Diameter of cap: 18.9 mm.

Wall thickness: 1.3 mm.

Nominal volume: 6.0 mL.

Maximum volume: 6.5 mL.

Temperature resistance: up to 80 °C.

Pico Glass Vial - 7 mL

Our Pico Glass Vial™ is a low background,

borosilicate glass vial. High counting efficiencies

compared to standard size glass vials are

obtained with as little as 3 mL of LSC cocktail.

These vials feature low background and are non-

permeable to aromatic hydrocarbons.

Specifications

Height with cap: 57.3 mm.

Diameter: 16.7 mm.

Diameter of opening: 8.3 mm.

Diameter of cap:

15.3 mm.

Wall thickness: 0.9 mm.

Nominal volume: 7.0 mL.

Maximum volume: 8.0 mL.

Temperature resistance:

>100 °C.

Our miniature vials are uniquely designed for safer, more confident sample preparation. Our plastic vials are

manufactured from high-density polyethylene (HDPE) and are available with patented “anti-static” treatment.

Unique closure designs are used to ensure fast, easy and comfortable sample preparation.

www.perkinelmer.com

Pony Vial - 6 mL

Our Pony Vial™ is a miniature polyethylene vial

with a unique (push-on/twist-off cap) closure sys-

tem not available on any other screw cap designs.

Push-on cap provides rapid closure: for routine

analysis wher

e many vials have to be handled,

push-cap vials are real time savers.

Twist-off cap for safer reopening; the Pony Vial

is compatible with all small vial counters and

PerkinElmer Varisette

™ sample changers.

Specifications

Height with cap: 56.6 mm.

Diameter: 16.0 mm.

Diameter of opening: 12.5 mm.

Diameter of cap: 15.9 mm.

Wall thickness: 1.3 mm.

Nominal volume: 5.5 mL.

Maximum volume: 6.0 mL.

Temperature resistance: up to 80 °C.

Pony ‘Hang-In’ Vial - 6 mL

Our Pony ‘Hang-In’ Vial is a miniature polyethyl-

ene scintillation vial with all the features of the

Pony Vial, but with a different cap that allows it to

‘hang’ into a standard 20 mL LSC vial as a carrier.

Specifications

Height with cap:

56.6 mm.

Diameter: 16.0 mm.

Diameter of opening: 12.5 mm.

Diameter of cap: 19.0 mm.

Wall thickness: 1.3 mm.

Nominal volume: 5.5 mL.

Maximum volume: 6.0 mL.

Temperature resistance: up to 80 °C.

Polypropylene Vial for MicroBeta - 4 mL

Polypropylene Vial and Cap for MicroBeta are 45

mm in height and hold 4.0 mL of LSC cocktail. In

MicroBeta TriLux, they are counted in vertical

position using cassette 1450-117.

Pony Vials

Miniature Vials

Product Product No. Description

Pico Pro Vial - 4 mL 6000252 2,000/case

Economically packed. Caps packed separately.

6000253 2,000/case

Same as above, with exclusive Anti-Static treatment.

Pico Prias Vial - 6 mL 6000192 2,000/case

Economically packed. Caps packed separately.

6000193 2,000/case

Same as above, with exclusive Anti-Static treatment.

Pico ‘Hang-In’ Vial - 6 mL 6000186 2,000/case

Economically packed. Caps packed separately.

6000187 2,000/case

Same as above, with exclusive Anti-Static treatment.

Pico Glass Vial - 7 mL 6000167 1,000/case

Shrink-wrapped in 5 partitioned trays of 200

vials each. Foil-lined, white urea screw caps

packed separately.

Pony Vial - 6 mL 6000592 1,000/case

Shrink-wrapped in 5 partitioned trays of

200 vials each. Caps packed separately.

6000292 2,000/case

Economically packed. Caps packed separately.

6000293 2,000/case

Same as above, with exclusive Anti-Static treatment.

Pony ‘Hang-In’ Vial - 6 mL 6000286 2,000/case

Economically packed. Caps packed separately.

6000287 2,000/case

Same as above, with exclusive Anti-Static treatment.

Polypropylene Vial for MicroBeta - 4 mL 1200-421 3,000/case

Economically packed. Caps packed separately.

Pico Glass Vial Caps 6000179 1,000/case

White urea screw caps with foil liner.

Miniature Vials Ordering Guide

www.perkinelmer.com

Mid-Sized Vials

Hinge Cap Vial - 8 mL

Our 8 mL capacity Hinge Cap Vial™, made from

high-density polyethylene (HDPE), is a revolution

in sample preparation for liquid scintillation

counting. Simply prepare your samples and close

the cap; the integral hinge fits flush with the vial

for snag-free counting in miniature vial cassettes.

Fast, Easy and Efficient Sample Preparation

33% more capacity than 6 mL miniature vials:

allows miniaturization from 20 mL size vials

when used with high sample capacity cocktails

such as Ultima Gold XR.

Enables reduced cocktail consumption: reducing

the amount of waste produced and waste

disposal costs.

Pre-labeling of attached cap: avoids potential

sample mix-ups for GLP compliance.

Fits miniature vial cassettes.

Specifications

Height with cap: 59.0 mm.

Diameter: 17.5 mm.

Diameter of opening: 14.0 mm.

Diameter of cap: 16.7 mm.

Wall thickness: 1.3 mm.

Nominal volume: 8.0 mL.

Maximum volume: 9.0 mL.

Temperature resistance:

up to 80 °C.

Midi-Vial - 8 mL

Our Midi-Vial™ is an 8 mL HDPE vial, providing

33% more sample capacity than miniature vials.

This allows miniaturization from 20 mL size vials

when used in combination with high sample

capacity cocktails such as Ultima Gold XR. It

features the same unique push-on/twist-off

closure system as Pony V

ials.

Specifications

Height with cap:

62.0 mm.

Diameter: 17.5 mm.

Diameter of opening: 14.0 mm.

Diameter of cap: 17.4 mm.

Wall thickness: 1.9 mm.

Nominal volume: 8.0 mL.

Maximum volume: 8.0 mL.

Temperature resistance: up to 80 °C.

Maxi-Vial - 18 mL

The large opening of our Maxi-Vial™ promotes

easy sample loading of filters and other sample

types. It features very low “classical” solvent dif-

fusion due to its thick 2 mm walls.

Specifications

Height with cap:

61.0 mm.

Diameter: 26.5 mm.

Diameter of opening: 22.6 mm.

Diameter of cap: 26.9 mm.

Wall thickness: 2 mm.

Nominal volume: 18.0 mL.

Maximum volume: 20.0 mL.

Temperature resistance: up to 80 °C.

Product Product No. Description

Hinge Cap Vial - 8 mL 6000480 2,000/case. Economically packed. Caps packed separately.

6000488 500/case. Shrink-wrapped in 5 partitioned trays of 100 vials each.

Midi-Vial - 8 mL 6000288 2,000/case. Economically packed. Caps packed separately.

6000289 2,000/case. Same as above, with exclusive Anti-Static treatment.

Maxi-Vial - 18 mL 6000201 1,000/case. Economically packed. Caps packed separately.

6000203 1,000/case. Same as above, with exclusive Anti-Static treatment.

High Performance

Glass Vials

Standard Vials

Super Polyethylene Vial with

Glass Vial Thread - 20 mL

Our Super Polyethylene Vial™ has excellent

mechanical strength and a seamless bottom and

walls. The glass vial thread enables use of caps

for 20 mL glass vials.

Specifications

Height with cap:

60.8 mm.

Diameter: 27.0 mm.

Diameter of opening: 17.5 mm.

Diameter of cap: 24.9 mm.

Wall thickness: 1 mm.

Nominal volume: 20.0 mL.

Maximum volume: 24.0 mL.

Temperature resistance: up to 80 °C.

Super Polyethylene Vial with

Quick Closure - 20 mL

This Super Polyethylene Vial features a polyeth-

ylene quick closure screw cap with a smooth grip.

Specifications

Height with cap: 60.8 mm.

Diameter: 27.0 mm.

Diameter of opening:

17.5 mm.

Diameter of cap: 24.7 mm.

Wall thickness: 1 mm.

Nominal volume: 20.0 mL.

Maximum volume: 24.0 mL.

Temperature resistance: up to 80 °C.

Low Diffusion Polyethylene Vial - 20 mL

Our Low Diffusion Polyethylene Vial carries a

micron thin Teflon

-type coating on the inside

surface, reducing the diffusion of classical type

solvents by a factor of 10–20 times. The cap is

lined with aluminum foil as a barrier. This vial

was developed for long-term low-level measure-

ments as a cost effective alternative to very

expensive Teflon

counting vials. They are 100%

anti-static and provide high counting efficiency

and low background.

Specifications

Height with cap:

60.8 mm.

Diameter: 27.0 mm.

Diameter of opening: 17.5 mm.

Diameter of cap: 24.9 mm.

Wall thickness: 1 mm.

Nominal volume: 20.0 mL.

Maximum volume: 24.0 mL.

Temperature resistance: up to 80 °C.

High Performance Glass Vial - 20 mL

Our High Performance Glass Vial™ is made from

specially selected low potassium borosilicate

glass and provides high UV transmission (≥90%).

The controlled low and stable background

assures batch-to-batch homogeneity. They are

supplied with a white cap with a good writing

surface in dust-free tray packaging.

Specifications

Height with cap:

60.5 mm.

Diameter: 27.3 mm.

Diameter of opening: 16.2 mm.

Diameter of cap: 24.9 mm.

Wall thickness: 0.9 mm.

Nominal volume: 20.0 mL.

Maximum volume: 24.0 mL.

Temperature resistance: >100 °C.

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Econo

Glass Vials

Econo Glass Vial - 20 mL

Our Econo Glass vial is made from standard

borosilicate glass, specially selected for accept-

able background. They are economical and

provide excellent counting performance.

Specifications

Height with cap:

60 mm.

Diameter: 27.3 mm.

Diameter of opening: 16.2 mm.

Diameter of cap: 24.9 mm.

Wall thickness: 0.9 mm.

Nominal volume: 20.0 mL.

Maximum volume: 22.0 mL.

Temperature resistance: up to 80 °C.

Oximate Vial - 20 mL

Our Oximate Vial™ is made of polyethylene or

glass and features a special cap design for use in

PerkinElmer’

s Sample Oxidizer.

Specifications

Height with cap:

60.8 mm.

Diameter: 27.3 mm.

Diameter of opening: 16.2 mm.

Diameter of cap: 24.9 mm.

Wall thickness: 0.9 mm.

Nominal volume: 20.0 mL.

Maximum volume: 24.0 mL.

Temperature resistance: >100 °C.

Product Product No. Description

Super Polyethylene Vial with 6001085 500/case

Glass Vial Thread - 20 mL Packed in 5 partitioned trays of 100 vials each with foil-lined urea screw caps on.

6001087 1,000/case

Economically packed. Foil-lined urea screw caps packed separately.

Super Polyethylene Vial with 6008117 1,000/case

Quick Closure - 20 mL Economically packed. Caps packed separately.

6008118 1,000/case

Same as above, with exclusive Anti-Static treatment.

6000375 500/case

Shrink-wrapped in 5 partitioned trays of 100 vials each. Caps packed separately.

6001075 500/case

Packed in 5 partitioned trays of 100 vials each with caps on.

Low Diffusion Polyethylene 6000477 100/case

Vial - 20 mL Shrink-wrapped in partitioned tray with caps on, with exclusive Anti-Static treatment.

Super Low Diffusion 1200-422 100/package

Polyvials - 20 mL Teflon

coated polyethylene caps included.

High Performance Glass 6001009 500/case

Vial - 20 mL Packed in 5 partitioned trays of 100 vials each with caps on.

Special design screw cap for use in automated handling.

6001015 500/case

Packed in 5 partitioned trays of 100 vials each with foil-lined screw caps on.

6001050 500/case

Packed in 5 partitioned trays of 100 vials each.

Poly-Cone lined urea screw caps packed separately.

6000349 500/case

Packed in 5 partitioned trays of 100 vials each with foil-lined urea screw caps on.

6000128 500/case

Shrink-wrapped in 10 partitioned trays of 50 vials each.

Foil-lined urea screw caps packed separately.

continued

Standard Vials Ordering Guide

Product Product No. Description

High Performance Glass 6000129 500/case

Vial - 20 mL (continued)

Shrink-wrapped in 5 partitioned trays of 100 vials each.

Foil-lined urea screw caps packed separately.

6000134 500/case

Shrink-wrapped in 10 partitioned trays of 50 vials each.

Poly-Cone lined urea screw caps packed separately.

6000135 500/case

Shrink-wrapped in 5 partitioned trays of 100 vials each.

Poly-Cone lined urea screw caps packed separately.

Econo Glass Vial - 20 mL 6000096 500/case

Shrink-wrapped in 4 partitioned trays of 125 vials each.

Foil-lined urea screw caps packed separately.

6000097 500/case

Shrink-wrapped in 4 partitioned trays of 125 vials each.

Poly screw caps packed separately.

6000098 500/case

Shrink-wrapped in 4 partitioned trays of 125 vials each.

Poly-Cone lined urea screw caps packed separately.

Econo Glass Vial - 20 mL 6000326 500/case

Shrink-wrapped in 5 partitioned trays of 100 vials each.

Foil-lined urea screw caps packed separately.

6000327 500/case

Shrink-wrapped in 5 partitioned trays of 100 vials each.

Poly screw caps packed separately.

6000348 500/case

Shrink-wrapped in 5 partitioned trays of 100 vials each.

Poly-Cone lined urea screw caps packed separately.

Glass Vials, low K-40 1210-131 500/package

content - 20 mL

Oximate Vial - 20 mL 6001095 500/case

Polyethylene vials with glass vial thread. Packed in 5 partitioned trays of

100 vials each with foil-lined urea screw caps on. Special design screw cap

for automated handling.

6001009 500/case

Glass vials. Packed in 5 partitioned trays of 100 vials each with foil-lined urea

screw caps on. Special design screw cap for automated handling.

Glass Vial Caps 6001025 1,000/case

Foil-lined urea screw caps.

6001027 1,000/case

Poly-Cone lined urea screw caps.

6000249 1,000/case

Poly screw caps for glass vials.

6000250 1,000/case

Foil-lined urea screw caps. Special design for automated handling.

Standard Vials Ordering Guide (continued)

Standard Vials

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QUANTULUS Vials

Teflon

Vials

Our Teflon

vials give the best performance and are ideal particularly for low level

C dating where benzene

is used. The Teflon

vials can be used with PerkinElmer’s QUANTULUS Ultra Low Level Liquid Scintillation

Spectrometer.

Product Product No. Description

Teflon

Vials - 3 mL 1220-500 10/package

Teflon

Vials - 7 mL 1220-501 10/package

Teflon

Vials - 15 mL 1220-502 10/package

Teflon

Vials - 20 mL 1220-503 10/package

Glass Vials Application Need Sample Capacity Vial Type Cat. No.

For applications requiring High Sample Capacity 18–20 mL High Performance Glass 6001009

optical clarity and/or the 6001015

use of aggressive or reactive 6001050

reagents 6000349

6000128

6000129

6000134

6000135

Oximate 6001095

6001009

Econo Glass 6000096

6000097

6000098

6000326

600327

6000348

Miniaturization 6–8 mL Pico Glass 6000167

Vials Selection Guide

Plastic Vials Application Need Sample Capacity Vial Type Cat. No.

For applications requiring High Sample Capacity 18–20 mL Maxi- 6000201

shatterproof and/or 6000203

combustible vials and for

Super Polyethylene 6001085

low level counting*

6001087

6008118

6000375

6001075

Low Diffusion Polyethylene 6000477

Miniaturization 4–8 mL Pony 6000582

6000292

6000293

Pony “Hang-In” 6000286

6000287

Pico Pro 6000252

6000253

Pico Prias 6000192

6000193

Pico “Hang-In” 6000186

Midi- 6000288

6000289

Hinge Cap 6000480

6000488

Cell Harvesting 4 mL Pico Pro 6000252

* For low humidity conditions, select vials with our exclusive Anti-Static treatment.

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Liquid Scintillation Cocktail Selection Guide by Filter Type

Filter-Count Ultima Gold F Ultima Gold MV Soluene-350 + Filter-Count +

Filter Type Hionic-Fluor Ultima Gold AB

Dry ✔✔ ✔ ✔

Glass Fiber Wet ✔✔✔

Dissolved

Dry ✔✔

Cellulose Nitrate Wet ✔

Dissolved ✔✔

Dry ✔✔ ✔ ✔

Cellulose Acetate Wet ✔✔✔

Dissolved ✔

Dry ✔✔

Mixed Cellulose Esters Wet ✔

Dissolved ✔✔

Dry ✔ ✔

PVC Wet ✔

Dissolved ✔✔

Dry ✔✔ ✔ ✔

Polyacrylonitrile Wet ✔✔✔

Dissolved

Dry ✔✔ ✔ ✔

Polycarbonate Wet ✔✔✔

Dissolved

Dry ✔✔ ✔ ✔

Teflon

Wet ✔✔✔

Dissolved

Dry ✔✔ ✔ ✔

Nylon Wet ✔ ✔ ✔

Dissolved

Dry ✔✔ ✔ ✔ ✔

PET Wet ✔✔✔✔

Dissolved

Dry ✔✔ ✔ ✔ ✔

Normal Paper Wet ✔✔✔✔

Dissolved

Liquid Scintillation Cocktails Selection Guide by Sample Type

SAMPLE CLASSIFICATION

Organic Aqueous, pH >8 Aqueous, pH 6–8, <0.1 molar Aqueous, pH <6

SAMPLE TREATMENT

SAMPLE TREATMENT SAMPLE TREATMENT SAMPLE TREATMENT

Ultima Gold AB (#6013309) Pico-Fluor 40 (#6013349)

• Up to 3 mL in 10 mL of cocktail for 0–1 M mineral acids • Up to 2 mL in 10 mL of cocktail for 0–1 M mineral acids

• Up to 2 mL in 10 mL of cocktail for 1–2 M mineral acids • Up to 1 mL in 10 mL of cocktail for 1–2 M mineral acids

Ultima Gold LLT (#6013377) Hionic-Fluor (#6013319)

• Up to 3 mL in 10 mL of cocktail for 0–1 M mineral acids •

Up to 1.5 mL in 10 mL of cocktail for 0–1 M mineral acids

• Up to 2 mL in 10 mL of cocktail for 1–2 M mineral acids • Up to 3 mL in 10 mL of cocktail for 1–2 M mineral acids

OptiPhase HiSafe 3 (#1200–437)

• Up to 3 mL in 10 mL cocktail for 0–1 M mineral acids

• Up to 3 mL in 10 mL cocktail for 1–2 M mineral acids

Ultima Gold (#6013329) Pico-Fluor 15 (#6013059)

• Up to 3 mL in 10 mL of cocktail • Up to 2 mL in 10 mL of cocktail

Ultima Gold MV (#6013159) Insta-Gel Plus (#6013359)

• Up to 2 mL in 10 mL of cocktail • Up to 2 mL in 10 mL of cocktail

Opti-Fluor (#6013199)

• Up to 2.5 mL in 10 mL of cocktail

Emulsifier Safe (#6013389)

• Up to 2 mL in 10 mL of cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 3 mL in 10 mL of cocktail

Ultima Gold (#6013329) Pico-Fluor 40 (#6013349)

• Up to 2.5 mL in 10 mL cocktail for 0–1 M alkalis • Up to 2 mL in 10 mL cocktail for 0–1 M alkalis

• Up to 1 mL in 10 mL cocktail for 1–2 M alkalis • Up to 1 mL in 10 mL cocktail for 1–2 M alkalis

OptiPhase HiSafe 2 (#1200-436) Hionic-Fluor (#6013319)

• Up to 2.5 mL in 10 mL cocktail for 0–1 M alkalis • Up to 1.5 mL in 10 mL cocktail for 0–1 M alkalis

• Up to 1 mL in 10 mL cocktail for 1–2 M alkalis • Up to 1 mL in 10 mL cocktail for 1–2 M alkalis

Ultima Gold F (#6013179) Insta-Fluor Plus (#6013127)

• Up to 10 mL in 10 mL cocktail • Up to 10 mL in 10 mL cocktail

Opti-Fluor O (#6013339)

• Up to 10 mL in 10 mL cocktail

OptiScint HiSafe (#1200-434)

• Up to 10 mL in 10 mL cocktail

BetaPlate Scint (#1205-440)

• Up to 10 mL in 10 mL cocktail

Direct addition Direct addition Direct addition Direct addition

Sample Type: Safer Cocktails Classical Cocktails

Liquid

www.perkinelmer.com

SAMPLE CLASSIFICATION

Organic Aqueous, pH >8 Aqueous, pH 6–8, <0.1 molar Aqueous, pH <6

SAMPLE TREATMENT

SAMPLE TREATMENT SAMPLE TREATMENT SAMPLE TREATMENT

Ultima Gold AB (#6013309) Pico-Fluor 40 (#6013349)

• Up to 3 mL in 10 mL of cocktail for 0–1 M mineral acids • Up to 2 mL in 10 mL of cocktail for 0–1 M mineral acids

• Up to 2 mL in 10 mL of cocktail for 1–2 M mineral acids • Up to 1 mL in 10 mL of cocktail for 1–2 M mineral acids

Ultima Gold LLT (#6013377) Hionic-Fluor (#6013319)

• Up to 3 mL in 10 mL of cocktail for 0–1 M mineral acids •

Up to 1.5 mL in 10 mL of cocktail for 0–1 M mineral acids

• Up to 2 mL in 10 mL of cocktail for 1–2 M mineral acids • Up to 3 mL in 10 mL of cocktail for 1–2 M mineral acids

OptiPhase HiSafe 3 (#1200–437)

• Up to 3 mL in 10 mL cocktail for 0–1 M mineral acids

• Up to 3 mL in 10 mL cocktail for 1–2 M mineral acids

Ultima Gold (#6013329) Pico-Fluor 15 (#6013059)

• Up to 3 mL in 10 mL of cocktail • Up to 2 mL in 10 mL of cocktail

Ultima Gold MV (#6013159) Insta-Gel Plus (#6013359)

• Up to 2 mL in 10 mL of cocktail • Up to 2 mL in 10 mL of cocktail

Opti-Fluor (#6013199)

• Up to 2.5 mL in 10 mL of cocktail

Emulsifier Safe (#6013389)

• Up to 2 mL in 10 mL of cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 3 mL in 10 mL of cocktail

Ultima Gold (#6013329) Pico-Fluor 40 (#6013349)

• Up to 2.5 mL in 10 mL cocktail for 0–1 M alkalis • Up to 2 mL in 10 mL cocktail for 0–1 M alkalis

• Up to 1 mL in 10 mL cocktail for 1–2 M alkalis • Up to 1 mL in 10 mL cocktail for 1–2 M alkalis

OptiPhase HiSafe 2 (#1200-436) Hionic-Fluor (#6013319)

• Up to 2.5 mL in 10 mL cocktail for 0–1 M alkalis • Up to 1.5 mL in 10 mL cocktail for 0–1 M alkalis

• Up to 1 mL in 10 mL cocktail for 1–2 M alkalis • Up to 1 mL in 10 mL cocktail for 1–2 M alkalis

Ultima Gold F (#6013179) Insta-Fluor Plus (#6013127)

• Up to 10 mL in 10 mL cocktail • Up to 10 mL in 10 mL cocktail

Opti-Fluor O (#6013339)

• Up to 10 mL in 10 mL cocktail

OptiScint HiSafe (#1200-434)

• Up to 10 mL in 10 mL cocktail

BetaPlate Scint (#1205-440)

• Up to 10 mL in 10 mL cocktail

Direct addition Direct addition Direct addition Direct addition

Sample Type: Safer Cocktails Classical Cocktails

Solid (soluble)

SAMPLE CLASSIFICATION

Non-digestible Sample on filter Digestible

SOLUBILIZER SOLUBILIZER SOLUBILIZER

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommendations Filter-Count (#6013149)

Ultima Gold MV (#6013159) Pico-Fluor 15 (#6013059)

No recommendations (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729)

then Permafluor E

(#6013187)

Combust in a Not soluble Soluble in

Soluene-350

SOLVABLE

PerkinElmer in organic organic

(#60003038)

(#6NE9100)

Sample Oxidizer solvent solvent

SAMPLE CLASSIFICATION

Plasma/Serum

SAMPLE TREATMENT

Ultima Gold (#6013329) No recommendations

• Up to 1 mL in 10 mL cocktail

Ultima-Flo M (#6013579)

• Up to 2 mL in 10 mL of cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 1 mL in 10 mL of cocktail

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommenda

tions (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729)

then Permafluor E

(#6013187)

Oxidation Solubilization, Solubilization, Direct addition

using

Soluene-350

using SOLVABLE

(#60003038)

(#6NE9100)

Liquid Scintillation Cocktails Selection Guide by Sample Type

Sample Type: Safer Cocktails Classical Cocktails

Solid (insoluble)

Sample Type: Safer Cocktails Classical Cocktails

Biological

www.perkinelmer.com

SAMPLE CLASSIFICATION

Feces Urine Milk Whole blood

SAMPLE TREATMENT

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommenda

tions (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729)

then Permafluor E

(#6013187)

Opti-Fluor (#6013199) Pico-Fluor 40 (#6013349)

• Up to 4.5 mL in 10 mL of cocktail • Up to 2.5 mL in 10 mL of cocktail

Ultima Gold (#6013329) No recommendations

• Up to 8 mL in 10 mL of cocktail

Ultima Gold LLT (#6013377)

• Up to 10 mL in 10 mL of cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 5 mL in 10 mL of cocktail

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommendations (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729)

then Permafluor E

(#6013187)

Oxidation Solubilization, Solubilization,

using

Soluene-350

using SOLVABLE

(#60003038)

(#6NE9100)

Oxidation Solubilization, Solubilization,

using

Soluene-350

using SOLVABLE

(#60003038)

(#6NE9100)

Direct additionDirect addition

Sample Type: Safer Cocktails Classical Cocktails

Biological

SAMPLE CLASSIFICATION

Plant Tissue

SAMPLE TREATMENT SAMPLE TREATMENT

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommendations (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729) then

Permafluor E

(#6013187)

Ultima Gold (#6013329) No recommendations

• Up to 2 mL of SOLVABLE in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436)

• Up to 2 mL of SOLVABLE in 10 mL cocktail

Ultima Gold (#6013329) Hionic-Fluor (#6013319)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

OptiPhase HiSafe 2 (#1200-436) Pico-Fluor 40 (#6013349)

• Up to 1 mL of Soluene-350 in 10 mL cocktail • Up to 4 mL of Soluene-350 in 10 mL cocktail

No recommendations (

H): Monophase S (#6013109)

(

C): Carbo-Sorb E (#6013729)

then Permafluor E

(#6013187)

Oxidation Solubilization, Solubilization,

using

Soluene-350

using SOLVABLE

(#60003038)

(#6NE9100)

Oxidation Solubilization, Solubilization,

using

Soluene-350 using SOLVABLE

(#60003038)

(#6NE9100)

SAMPLE CLASSIFICATION

Radon Carbon dioxide

N/A TRAPPING AGENT

No recommendations Perma-Fluor E

(#6013187)

Emulsifier Safe (#6013389) Hionic-Fluor (#6013319)

Opti-Fluor (#6013199)

No recommendations Insta-Fluor Plus (#6013127)

High Efficiency Mineral Oil Scintillator (#6NE9579) Insta-Fluor Plus (#6013127)

Ultima Gold F (#6013179)

Opti-Fluor O (#6013339)

BetaPlate Scint (#1205-440)

N/A Hyamine NaOH Carbo-Sorb E

Hydroxide

(#6013729)

(#6003005)

Liquid Scintillation Cocktails Selection Guide by Sample Type

Sample Type: Safer Cocktails Classical Cocktails

Biological

Sample Type: Safer Cocktails Classical Cocktails

Gas

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HPLC Gradient Safer Cocktails Classic Cocktails

Water/Methanol Gradients Ultima-Flo M (#6013579) Flo-Scint III (#6013539)

Ultima-Flo AP (#6013599)

Water/Acetonitrile Gradients Ultima-Flo M (#6013579) Flo-Scint A (#6013569)

Flo-Scint III (#6013539)

Aqueous Gradients (0.1–2 M) Ultima-Flo AP (#6013599) Flo-Scint II (#6013529)

0–2 M Ammonium Phosphate Gradients Ultima-Flo AP (#6013599) No recommendations

0–2 M Ammonium Formate Gradients Ultima-Flo AF (#6013589) No recommendations

Ultima-Flo AP (#6013599)

Dilute Aqueous (0–0.1 M) Ultima-Flo M (#6013579) Flo-Scint II (#6013529)

Ultima-Flo AP (#6013599) Flo-Scint III (#6013539)

Organic No recommendations Flo-Scint A (#6013569)

Insta-Fluor Plus (#6013127)

Narrowbore/Microbore Ultima-Flo M (#6013579) Flo-Scint III (#6013539)

Flow Cocktails Selection Guide

Sample Preparation Guide

This table provides an overview of the sequential steps necessary for optimum sample preparation

for various types of applications. Data presented are typical for the indicated samples.

Abbreviations:

Tritium counting efficiency was determined on a PerkinElmer Tri-Carb Model 3100TR with 65% efficiency.

IPA = Isopropanol

TLC = Thin layer chromatogram

Sample: Procedural Steps

Biological #1 #2 #3

Fluids

Blood Add 1 mL mixture of Soluene-350: Stand 2 h at 60 °C. Add 0.2–0.5 mL 30% H

IPA

(1.1) to 0.1–0.4 mL blood. dropwise with swirling.

Add 1 mL SOLVABLE to 0.1–0.5 mL Stand 1 h at 60 °C. Add 0.1 mL 0.1 M EDTA-

blood. Na

solution.

Add 0.3–0.5 mL

30% H

in aliquots.

Plasma Add up to 1 mL plasma or serum Shake until clear.

or Serum to 10–15 mL of Ultima Gold.

Red Blood Add 1 mL Soluene-350:IPA mixture Stand 1 h at 60 °C. Add 0.2–0.5 mL 30% H

Cells (RBC) (1:1) to 0.2 mL RBC suspension. dropwise with swirling.

Add 1 mL SOLVABLE to 0.2 mL Stand 1 h at 60 °C. Add 0.1 mL 0.1 M EDTA-

RBC suspension. Na

solution.

Add 0.3–0.5 mL

30% H

in aliquots.

Urine Add up to 8 mL of urine to 12 mL of

Ultima Gold LLT and shake vigorously.

0.1 M Phosphate Add up to 8 mL to 10 mL Ultima Gold LLT

Buffered Saline (PBS) or up to 4 mL to 10 mL Ultima Gold or up

to 10 mL to 10 mL Ultima Gold XR.

Aqueous

Proteinaceous

Add 0.2 mL sample to 1 mL Soluene-350. Swirl until clear. Add 10 mL Hionic-Fluor.

Sample

Add 0.2 mL sample to 1 mL SOLVABLE. Swirl until clear or Add 10 mL Ultima Gold.

heat 30 min at 50 °C.

Sucrose Solutions Add between 3 mL and 7 mL of 20–40%

(w/v) sucrose to 10 mL Ultima Gold XR or

add between 5 mL and 10 mL of 30–60%

(w/v) sucrose to 10 mL Hionic-Fluor.

Inulin Containing Fluids Add 50 µL of inulin sample to 0.5 mL Add 10 mL of Hionic-Fluor.

of Soluene-350 and swirl.

Trichloroacetic Acid Add up to 3 mL of up to 20% TCA For concentrations over

(TCA) Supematant supernatant to 10 mL of Ultima Gold LLT. 20%, use Hionic-Fluor.

www.perkinelmer.com

For detailed information on sample uptake for PerkinElmer’s LSC cocktails for various types of

aqueous and organic liquids, please refer to the individual product descriptions.

Note: The PerkinElmer 307 Sample Oxidizer can easily be used to prepare up to 1–2 grams of any of the listed samples.

Recommended Expected

#4 #5 Sample Size

Efficiency

Stand 15–30 min ambient. Cap Add 10–15 mL Hionic-Fluor. Up to 0.4 mL blood 20–30%

tightly. Stand 30 min at 60 °C.

Stand 15–30 min ambient. Cap Add 10–15 mL Ultima Gold. Up to 0.5 mL blood 25–35%

tightly. Stand 30 min at 60 °C.

Up to 1 mL 30–40%

Stand 15–30 min ambient. Cap Add 10–15 mL Hionic-Fluor. Up to 0.2 mL RBC suspension 20–30%

tightly. Stand 30 min at 60 °C.

Stand 15–30 min ambient. Cap Add 10–15 mL Ultima Gold. Up to 0.2 mL RBC suspension 25–35%

tightly. Stand 30 min at 60 °C.

Up to 8 mL 25–35%

Up to 10 mL 30–40%

Up to 0.2 mL 35–40%

Up to 10 mL 30–40%

Up to 50 µL 40–50%

Up to 3 mL 25–40%

Sample Preparation Guide

Sample: Procedural Steps

Biological #1 #2 #3

Tissues

Homogenate Add 0.2 mL of up to 10% tissue Add 10 mL of Shake vigorously.

homogenate (in either water or 70% Insta-Gel Plus.

ethanol) to 3 mL of water.

Coarse-Ground Tissue Add 150 mg coarse-ground tissue to Stand for 3–5 h at 60 °C. Add 10 mL Hionic-Fluor.

2 mL Soluene-350 and swirl.

Add 150 mg coarse-ground tissue to Stand for 3–5 h at 60 °C. Add 10 mL Ultima Gold.

2 mL SOLVABLE and swirl.

Bacteria and Cells Add 1 mL of 8:2 Soluene-350:water to Stand 2–4 h at 60 °C. Add 10 mL Hionic-Fluor.

5–7 mg of bacteria or cells.

Add 1 mL of SOLVABLE to 5–7 mg Stand 2–4 h at 60 °C. Add 10 mL Ultima Gold.

of bacteria or cells.

Organs Add 1 mL Soluene-350 per: Stand 2–4 h at 60 °C. Add 10 mL Hionic-Fluor.

Arteries: 30–100 mg Intestine: 80–100 mg Pancreas: 50–110 mg

Brain: 50–150 mg Kidney: 50–100 mg Spleen: 50–140 mg

Cartilage: 20–55 mg Liver: 50–100 mg Stomach: 50–100 mg

Cornea: 40–160 mg Muscle: 100–200 mg Sinew: 50–150 mg

Heart: 50–100 mg Nerve cells: 50–100 mg

Add 1 mL SOLVABLE per: Stand 2–4 h at 60 °C. Add 10 mL Ultima Gold.

Arteries: 30–100 mg Intestine: 80–100 mg Pancreas: 50–110 mg

Brain: 50–150 mg Kidney: 50–100 mg Spleen: 50–140 mg

Cartilage: 20–55 mg Liver: 50–100 mg Stomach: 50–100 mg

Cornea: 40–160 mg Muscle: 100–200 mg Sinew: 50–150 mg

Heart: 50–100 mg Nerve cells: 50–100 mg

Feces Add 0.1 mL water to 20 mg dried feces; Add 1 mL Soluene–350. Stand 1–2 h at 50 °C.

rehydrate for 30 min. Add 1 mL IPA and mix.

Stand 2 h at 50 °C.

Add 0.1 mL water to 20 mg dried feces; Add 1 mL SOLVABLE. Stand 1–2 h at 50 °C.

rehydrate for 30 min. Add 1 mL IPA and mix.

Stand 2 h at 50 °C.

TLC

-Scrapings Add water-soluble sample on TLC silica Stand 3–5 h at 40 °C. Add Note: If samples are not

to 1 mL of H

O. 8–10 mL of Insta-Gel Plus. water soluble, add 1 mL of

Soluene-350 instead of H

Polyacrylamide Gel Add 1–2 mm gel slice to 0.5 mL Stand for 3 h at 50 °C. Add 10 mL Hionic-Fluor.

Slices (PAGE) Soluene-350.

Add 1–2 mm gel slice to 0.5 mL Stand for 3 h at 50 °C. Add 10 mL Ultima Gold.

SOLVABLE.

TCA Precipitates Moisten 100 mg of dried TCA precipitate Rehydrate for 30 min. Add 1 mL Soluene-350

(proteinaceous) with 0.1–0.2 mL water. and stand 30 min ambient.

Moisten 100 mg of dried TCA precipitate Rehydrate for 30 min. Add 1 mL SOLVABLE

(proteinaceous) with 0.1–0.2 mL water. and stand 30 min ambient.

Filters Place filter on bottom of vial. Add 0.5–1.0 mL Add 10 mL Hionic-Fluor.

(Cellulose acetate only) Rehydrate with 0.1–0.2 mL H

O. Soluene-350. Stand for

30 min ambient.

Place filter on bottom of vial. Add 5–10 mL Filter-Count.

Drying wet filters is not required. Shake several times until

filter is dissolved and count.

Abbreviations:

Tritium counting efficiency was determined on a PerkinElmer Tri-Carb Model 3100TR with 65% efficiency.

IPA = Isopropanol

TLC = Thin layer chomatogram

www.perkinelmer.com

Recommended Expected

#4 #5 Sample Size

Efficiency

Note: Homogenates can Up to 0.2 mL of 10% 30–40%

also be prepared as for tissue homogeneate

coarse-ground tissue.

Up to 1.0 mL of 10% tissue 35–40%

homogenate. Up to 150 mg

coarse-ground tissue.

Up to 1.0 mL of 10% tissue 35–45%

homogenate. Up to 150 mg

coarse-ground tissue.

5–7 mg of bacteria or cells 20–30%

5–7 mg of bacteria or cells 35–45%

See Steps 1, 2 and 3. 17–40%

See Steps 1, 2 and 3. 30–45%

Add 0.2 mL of 30% H

Stand 10 min ambient; Up to 20 mg dried feces 25–35%

dropwise with swirling. add 10 mL Hionic-Fluor.

Add 0.2 mL of 30% H

Stand 10 min ambient; 30–40%

dropwise with swirling. add 10 mL Hionic-Fluor.

Stand 3–5 h at 40 °C. 30–40%

Add 10 mL of Hionic-Fluor.

1–2 mm gel slice 45–50%

1–2 mm gel slice 50–55%

Add 10 mL Hionic-Fluor. Up to 100 mg 35–40%

Add 10 mL Ultima Gold. Up to 100 mg 40–50%

50–55%

35–45%

Note: The PerkinElmer 307 Sample Oxidizer can easily be used to prepare up to 1–2 grams of any of the listed samples.

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices

Safe, Filter

-Count, High Performance Glass Vial, Hinge Cap Vial, Hionic-Fluor, Insta-Fluor, Maxi-Vial, MicroScint, Midi-Vial, OptiScint,

OptiSolv, Oximate Vial, Pico-Fluor, Pico Glass Vial, Pico 'Hang-In' Vial, Pico Prias Vial, Pico Pro Vial, Pony Vial, SOLVABLE, Super

Polyethylene Vial, Ultima-Flo, Ultima Gold, and Varisette are trademarks and Atomlight, Biofluor, Carbo-Sorb, En

Hance, ENLIGHTNING,

Formula-989, HiSafe, NEN, Opti-Fluor, Meltilex, MicroBeta, Monophase, Permafluor, QUANTULUS, Soluene, TopCount and Tri-Carb are

registered trademarks of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. LIPIDEX is a registered trademark

of DL Industries. OptiPhase is a trademark of Fisons Chemicals. Sephadex is a registered trademark of Pharmacia Laboratories, Inc. Teflon

is a registered trademark of E.I. du Pont de Nemours and Company. The Hydroxide of Hyamine is a registered trademark of Lonza. All

other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners.

PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or

typographical errors.

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